Journal: Micromachines

Article Title: Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

doi: 10.3390/mi12030346

Figure Lengend Snippet: The biocompatible adhesive microchip fabrication is based on using a double-sided biocompatible adhesive to join the top and bottom layers. Top layer: glass slide, coverslip, or polyester). Middle layer: cut double-sided biocompatible adhesive. Bottom layer: glass slide, Permanox ® , or polystyrene film. The top of the microchip is a female luer glued to facilitate the silicone hose fitting for microfluidic operation.

Article Snippet: The bottom layer serves as a bed for the growth of the HUVEC; therefore, the materials with good cell adhesion and growth, such as Permanox ® slide (75 mm × 25 mm, Thermo Scientific Nunc, US), polystyrene slide (75 mm × 25 mm, obtained from cutting of 75 cm 2 cell culture flask, Corning, USA) or glass slide (76.2 mm × 25.4 mm with a thickness of 1 to 1.2 mm, Perfecta, Brazil) treated with 50 μL of fibronectin solution (fibronectin from bovine plasma, Sigma-Aldrich) at 10 μg mL −1 prevailed as suitable in this task.

Techniques: Adhesive, MicroChIP Assay

Journal: Micromachines

Article Title: Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

doi: 10.3390/mi12030346

Figure Lengend Snippet: Biocompatible adhesive microchips made of different top-bottom materials: ( A ) glass-glass; ( B ) coverslip-glass; ( C ) glass-Permanox ® ; ( D ) polyester-Permanox ® ; ( E ) glass-polystyrene; ( F ) polyester-polystyrene; ( G ) frontal and lateral section dimensions of biocompatible adhesive microchips illustrating the length (30 mm), the height (0.14 mm) and the width (2 mm) of the channel.

Article Snippet: The bottom layer serves as a bed for the growth of the HUVEC; therefore, the materials with good cell adhesion and growth, such as Permanox ® slide (75 mm × 25 mm, Thermo Scientific Nunc, US), polystyrene slide (75 mm × 25 mm, obtained from cutting of 75 cm 2 cell culture flask, Corning, USA) or glass slide (76.2 mm × 25.4 mm with a thickness of 1 to 1.2 mm, Perfecta, Brazil) treated with 50 μL of fibronectin solution (fibronectin from bovine plasma, Sigma-Aldrich) at 10 μg mL −1 prevailed as suitable in this task.

Techniques: Adhesive

Journal: Micromachines

Article Title: Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

doi: 10.3390/mi12030346

Figure Lengend Snippet: HUVEC (5 × 10 6 cells mL –1 ) proliferation after 4 h and 24 h of incubation at 37 °C with RPMI-1640 culture medium flow rate at 2 µL min −1 in the microchannel of biocompatible double-sided adhesive microchips made with top-bottom layers: ( A ) glass slide-glass slide, ( B ) coverslip-glass slide, ( C ) glass slide-Permanox ® , ( D ) polyester-Permanox ® , ( E ) glass-polystyrene slide, ( F ) polyester-polystyrene slide. The cell viability was detected by the mixture of AO and EB staining. Green dots correspond to live cells and red-orange dots correspond to dead cells. Scale bar = 200 µm.

Article Snippet: The bottom layer serves as a bed for the growth of the HUVEC; therefore, the materials with good cell adhesion and growth, such as Permanox ® slide (75 mm × 25 mm, Thermo Scientific Nunc, US), polystyrene slide (75 mm × 25 mm, obtained from cutting of 75 cm 2 cell culture flask, Corning, USA) or glass slide (76.2 mm × 25.4 mm with a thickness of 1 to 1.2 mm, Perfecta, Brazil) treated with 50 μL of fibronectin solution (fibronectin from bovine plasma, Sigma-Aldrich) at 10 μg mL −1 prevailed as suitable in this task.

Techniques: Incubation, Adhesive, Staining

Journal: Micromachines

Article Title: Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

doi: 10.3390/mi12030346

Figure Lengend Snippet: Comparative analyses for the cost of materials and the time for fabrication for each type of microchip produced at the laboratory scale capacity.

Article Snippet: The bottom layer serves as a bed for the growth of the HUVEC; therefore, the materials with good cell adhesion and growth, such as Permanox ® slide (75 mm × 25 mm, Thermo Scientific Nunc, US), polystyrene slide (75 mm × 25 mm, obtained from cutting of 75 cm 2 cell culture flask, Corning, USA) or glass slide (76.2 mm × 25.4 mm with a thickness of 1 to 1.2 mm, Perfecta, Brazil) treated with 50 μL of fibronectin solution (fibronectin from bovine plasma, Sigma-Aldrich) at 10 μg mL −1 prevailed as suitable in this task.

Techniques: MicroChIP Assay, Produced